Journal: Cell chemical biology

Article Title: Insulin sensitization by small molecules enhancing GLUT4 translocation

doi: 10.1016/j.chembiol.2023.06.012

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ProteomeXchange PXD034052 Structure of Unc119-inhibitor complex PDB dataset ID: D_1000264081 PDB ID: 7UMO Experimental models: Cell lines CHO-K1 ATCC CCL-61, RRID: CVCL_0214 C2C12 ATCC CRL-1772; RRID: CVCL_0188 HBG4 This paper N/A Experimental models: Organisms/strains C57BL/6 Jackson Laboratory RRID:IMSR_JAX: 000664 HBG4 This paper N/A Oligonucleotides gRNA targeting GLUT4: CTGGGTAGGCAAGGTCCTGG This paper N/A SLC2A4 forw: ACA GCT CTC AGG CAT CAA TG This paper N/A SLC2A4 rev: GTT CTA CTA AGA GCA CCG AGA This paper N/A TBC1D4 forw: CAT CTG TTA TGT GTT CCA GTG C This paper N/A TBC1D4 rev: CAG TTT AAT CTG CGT CTT GGC This paper N/A TBC1D1 forw: CTT ATT GAG TCC TCG CCT CTC This paper N/A TBC1D1 rev: GCT TTG GAT CCT AGC ATT TGC This paper N/A Actin forw: GAC TCA TCG TAC TCC TGC TTG This paper N/A Actin rev: GAT TAC TGC TCT GGC TCC TAG This paper N/A gRNA_1-Unc119: ACCAAGGCGGGACGGCA CCUGUUUUAGAGCUAUGCU This paper N/A gRNA-Unc119_2: GGAUCACGGGUGGUG AGCACGUUUUAGAGCUAUGCU This paper N/A Unc119 forw: GGTCAGGGAACAAGCAAGAA This paper N/A Unc119 rev: GGAGGCAAAGAGAGAGAGAGTA This paper N/A gRNA-Unc119b_1: AGCACGUCCUACGCCUC AACGUUUUAGAGCUAUGCU This paper N/A gRNA-Unc119b_2: ACCAAGAUGGCCGCCA CCUCGUUUUAGAGCUAUGCU This paper N/A Unc119b forw: GGGAACTTTCAGGATAGCATTTG This paper N/A Unc119b rev: GTTGGTCAGATCAGCAGGAT This paper N/A Recombinant DNA secLgBiT Adenovirus plasmid This paper N/A HiBiT-GLUT4-mCherry Lentivirus plasmid This paper N/A PPRE-X3-TK-Luc Addgene Plasmid #1015 Software and algorithms Prism 9 GraphPad N/A Persus Persus N/A X-ray detector XDS N/A Scala CCP4 program suite N/A PMOD software version 3.709 PMOD Technologies N/A Open in a separate window KEY RESOURCES TABLE.

Techniques: Virus, Recombinant, In Vivo, Plasmid Preparation, Software